trpa1 polyclonal antibody  (Proteintech)

Journal: Journal of Functional Foods

Article Title: A thermoprotective probiotic function by thermostable lactic acid bacteria and its causal structure

doi: 10.1016/j.jff.2024.106001

Figure Lengend Snippet: Fig. 7. Conceptual diagram estimated from this research. Panel (a) shows chickens and humans weakened by the heat, i.e., growth inhibition and quality of life (QOL). (b) It shows the control of hydroxylation of transient receptor potential ankyrin1 (TRPA1), which is a temperature sensitivity sensor by oxalic acid as previously reported (Miyake et al., 2016). The presence of oxalic acid has already been shown to inhibit proline phosphorylation of TRPA1, resulting in increased sensitivity to temperature. Prolyl hydroxylases (PHDs) are involved in this reaction. Red arrows indicate ion influx, green indicates hydroxylation reactions and blue lines indicate inhibitory effects. Dashed lines indicate that each intensity is weak rather than solid lines. (c) A putative model, showing a mechanism of action of W. coagulans under heat-stress conditions, was visualized. After treatment with W. coagulans, the level of mannitol in carbohydrate metabolism was increased, based on the interaction of the intestinal microbiota. The model then shows a decrease in Pseudomonas and an increase in pyridoxal in the metabolism of vitamin B6. The effect of this relationship was shown to be a reduction in oxalic acid in the liver. Furthermore, the decrease in oxalic acid may be related to PHDs (HIF-1α-specific prolyl hydroxylases), HIF (hypoxia inducer) 1α under hypoxia, reactive oxygen species (ROS) as oxidative stress, and transient receptor potential ankyrin 1 (TRPA1). These conditions control the irritability that causes a change in sensitivity to cold as previously reported (Miyake et al., 2016). The red arrows show the cascade of increased sensitivity to cold. Dashed lines, which mean that each intensity is weak rather than solid lines, show a weak cascade caused by the exposure of W. coagulans under heat-stress conditions of this study. The green arrows also show a putative cascade of decreased sensitivity to cold as a mechanism of action of W. coagulans under heat-stress conditions of this study. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: The membranes were blocked with PVDF Blocking Reagent (TOYOBO, Japan) at room temperature for 1 h. The primary antibodies were rabbit polyclonal TRPA1 antibody (19124-1-AP) (proteintech group, Japan) diluted 1:1500 or rabbit polyclonal HIF-1 alpha antibody (bs-20399R) (Bioss andibodies, USA) diluted 1:1500 and the secondary antibody was horseradish peroxidase-conjugated goat anti-rabbit IgG antibodies diluted 1:12000 (Cell Signaling Technology, USA).

Techniques: Inhibition, Control, Phospho-proteomics